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2023, Frontiers in Microbiology
Postbiotics, which are bioactive substances derived from the metabolic processes of beneficial microbes, have received considerable attention in the field of microbiome science in recent years, presenting a promising path for exploration and innovation. This comprehensive analysis looks into the multidimensional terrain of postbiotic production, including an extensive examination of diverse postbiotic classes, revealing their sophisticated mechanisms of action and highlighting future applications that might significantly affect human health. The authors thoroughly investigate the various mechanisms that support postbiotic production, ranging from conventional fermentation procedures to cutting-edge enzyme conversion and synthetic biology approaches. The review, as an acknowledgment of the field's developing nature, not only highlights current achievements but also navigates through the problems inherent in postbiotic production. In order to successfully include postbiotics in therapeutic interventions and the production of functional food ingredients, emphasis is given to critical elements, including improving yields, bolstering stability, and assuring safety. The knowledge presented herein sheds light on the expanding field of postbiotics and their potential to revolutionize the development of novel therapeutics and functional food ingredients.
AgroLife Scientific Journal
For a long time, probiotics have been widely used as safe microorganisms that can confers a health benefit effects on the host, directly or indirectly. Recently, postbiotics have gained interest as new health promoters. Postbiotics have recently been defined as complex mixture of functional bioactive compounds secreted by probiotics during a fermentation process (such as biosurfactants, proteins, short chain fatty acids, organic acids, bacteriocins, vitamins etc.). According to current data, postbiotics have advantages over live probiotics with regard to: ease extraction, standardization, and storage, availability for industrial-scale-up, specific mechanism of action, impossible to transfer and acquire antibiotic resistance genes and their interaction with the cellular receptors to trigger the targeted responses. However, several aspects related to postbiotics have not been fully elucidated. Here, we provided a critical review of the postbiotic definition, mechanisms of action, unde...
eFood - Wiley, 2022
It is a known fact that a number of routes explaining the health benefits of beneficial bacterial cells require vitality. However, more recent terms such as the para probiotic and postbiotic have appeared to indicate that, in addition to live cultures, nonviable microbial cells, microbial fractions, or cell lysates also have the ability to benefit the host by boosting bioactivity. According to current scientific research, postbiotics are potentially safer than their parent live cells as alternative agents. Because of their distinct clinical, technological, and economic properties, they can be used as promising tools in the food and drug industries to achieve therapeutic goals and health benefits. The postbiotic theory, proof of their health benefits, probable signaling pathways involved in their protective mechanisms, and their potential use as functional foods are all covered in this review. With the use of comparative studies on past research, it discusses the methodologies utilized to obtain and identify postbiotics. It educates about postbiotics' bioactivity and probable physiological effects. It also instructs of all possible food applications and underlines the processes involved.
Microbial Cell Factories, 2020
Probiotics have several health benefits by modulating gut microbiome; however, techno-functional limitations such as viability controls have hampered their full potential applications in the food and pharmaceutical sectors. Therefore, the focus is gradually shifting from viable probiotic bacteria towards non-viable paraprobiotics and/or probiotics derived biomolecules, so-called postbiotics. Paraprobiotics and postbiotics are the emerging concepts in the functional foods field because they impart an array of health-promoting properties. Although, these terms are not well defined, however, for time being these terms have been defined as here. The postbiotics are the complex mixture of metabolic products secreted by probiotics in cell-free supernatants such as enzymes, secreted proteins, short chain fatty acids, vitamins, secreted biosurfactants, amino acids, peptides, organic acids, etc. While, the paraprobiotics are the inactivated microbial cells of probiotics (intact or ruptured containing cell components such as peptidoglycans, teichoic acids, surface proteins, etc.) or crude cell extracts (i.e. with complex chemical composition)". However, in many instances postbiotics have been used for whole category of postbiotics and parabiotics. These elicit several advantages over probiotics like; (i) availability in their pure form, (ii) ease in production and storage, (iii) availability of production process for industrial-scale-up, (iv) specific mechanism of action, (v) better accessibility of Microbes Associated Molecular Pattern (MAMP) during recognition and interaction with Pattern Recognition Receptors (PRR) and (vi) more likely to trigger only the targeted responses by specific ligand-receptor interactions. The current review comprehensively summarizes and discussed various methodologies implied to extract, purify, and identification of paraprobiotic and postbiotic compounds and their potential health benefits.
Alborz University Medical Journal
Although for a long time it was thought that the health benefits of probiotics depend on their viability but, in the last decade, studies have shown that the passive form of these bacteria (non-viable), microbial fractions, and cell lysates also offer physiological benefits to the host. Thus from 2011 onwards, new terms such as postbiotic have emerged, indicating that viability is not necessary for health-promoting effects of probiotics. Postbiotics refers to soluble factors secreted by live bacteria or released after bacterial lysis including, enzymes, peptides, polysaccharides, organic acids, teichoic acids, and cell surface proteins. Treatment of some diseases in human and animals has been demonstrated in case of using these postbiotics. Identification of postbiotics can be associated with the production and development of drug formulations without live bacteria. With this review, we aim to provide a comprehensive picture of the postbiotics, their production and identification methods, and their application in food and pharrmaceutical industries.
International journal of scientific research and management, 2024
As an imbalance in the intestinal microbiota can lead to the development of several diseases (e.g., type 1 diabetes, cancer, among others), the use of prebiotics, probiotics, and postbiotics to alter the gut microbiome has attracted recent interest. Postbiotics include any substance released by or produced through the metabolic activity of the microorganism, which exerts a beneficial effect on the host, directly or indirectly. As postbiotics do not contain live microorganisms, the risks associated with their intake are minimized. Here, we provided a critical review of postbiotics described in the literature, including their mechanisms of action, clinical characteristics, and potential therapeutic applications. We detailed the pleiotropic effects of postbiotics, including their immunomodulatory, anti-inflammatory, antioxidant, and anti-cancer properties. Although the use of postbiotics is an attractive strategy for altering the microbiome, further study into its efficacy and safety is warranted.
SSRN Electronic Journal
Globally, value addition to food is one of the key objectives of nearly all food industries. Several technologies have been employed to enhance both the nutritional and sensory properties of foods. The useful effects of foods with probiotic microbes on human health have been progressively investigated and supported by health specialists since time immemorial. The use of probiotic bacteria in fermentation has been increasing tremendously in this current era, and there is scientific substantiation indicating the use of probiotic bacteria in health care delivery and agriculture. It has been applied in various food industries for fermentation to promote the safety of the product. Although there has been strong scientific substantiation associating the use of probiotic microorganisms in fermentation, health and agriculture, Proper documentation has not been presented. The purpose of this review is to document the insight of probiotic fermentation and the future of it for human kind in the literature, focusing on highlighting the benefits of probiotics in health and agriculture, and also some mechanisms of action of probiotics.
Current opinion in biotechnology, 2015
Nutraceuticals are important natural bioactive compounds that confer health-promoting and medical benefits to humans. Globally growing demands for value-added nutraceuticals for prevention and treatment of human diseases have rendered nutraceuticals a multi-billion dollar market. However, supply limitations and extraction difficulties from natural sources such as plants, animals or fungi, restrict the large-scale use of nutraceuticals. Metabolic engineering via microbial production platforms has been advanced as an eco-friendly alternative approach for production of value-added nutraceuticals from simple carbon sources. Microbial platforms like the most widely used Escherichia coli and Saccharomyces cerevisiae have been engineered as versatile cell factories for production of diverse and complex value-added chemicals such as phytochemicals, prebiotics, polysaccaharides and poly amino acids. This review highlights the recent progresses in biological production of value-added nutraceu...
Frontiers in Microbiology
The gut microbiome has been increasingly understood to play a critical role in carcinogenesis and cancer disease progression. The most recent research advancements have shown that different tools of microbiota manipulation contribute to gut microbiome–immune–oncology axis modulation, offering exciting opportunities for targeted interventions aimed at improving the efficacy of established anti-cancer therapy. Postbiotics are a new entry among the biotics showing beneficial effects on human health while not requiring living cells to obtain the health effect and therefore not subjected to food safety rules for live microorganisms. Postbiotics are recently defined as the “preparation of inanimate microorganisms and/or their components that confers a health benefit on the host” and have gradually become the focus of the scientific community. Since the beginning of research on this topic, numerous studies about postbiotics have been proven to strengthen the gut barrier, reduce inflammatio...
Trends in Microbiology, 2021
Recent and ongoing developments in microbiome science are enabling new frontiers of research for probiotics and prebiotics. Novel types, mechanisms, and applications currently under study have the potential to change scientific understanding as well as nutritional and healthcare applications of these interventions. The expansion of related fields of microbiome-targeted interventions, and an evolving landscape for implementation across regulatory, policy, prescriber, and consumer spheres, portends an era of significant change. In this review we examine recent, emerging, and anticipated trends in probiotic and prebiotic science, and create a vision for broad areas of developing influence in the field. Highlights An expanding range of candidate probiotic species and prebiotic substrates is emerging to address newly elucidated data-driven microbial niches and host targets. Overlapping with, and adjacent to, the probiotic and prebiotic fields, new variants of microbiome-modulating interventions are developing, including synbiotics, postbiotics, microbial consortia, live biotherapeutic products, and genetically modified organisms, with renewed interest in polyphenols, fibres, and fermented foods. Personalised nutrition and precision medicine are beginning to influence the application of probiotics and prebiotics, with growing interest in modulation of microbial signatures of health and disease. Demand for probiotics and prebiotics across divergent product formats is driving innovation in quality assurance techniques to measure dose, viability, and structural and functional integrity.
Nutrients, 2020
As an imbalance in the intestinal microbiota can lead to the development of several diseases (e.g., type 1 diabetes, cancer, among others), the use of prebiotics, probiotics, and postbiotics to alter the gut microbiome has attracted recent interest. Postbiotics include any substance released by or produced through the metabolic activity of the microorganism, which exerts a beneficial effect on the host, directly or indirectly. As postbiotics do not contain live microorganisms, the risks associated with their intake are minimized. Here, we provided a critical review of postbiotics described in the literature, including their mechanisms of action, clinical characteristics, and potential therapeutic applications. We detailed the pleiotropic effects of postbiotics, including their immunomodulatory, anti-inflammatory, antioxidant, and anti-cancer properties. Although the use of postbiotics is an attractive strategy for altering the microbiome, further study into its efficacy and safety i...
Frontiers in Sustainable Food Systems
The microbiome innovation has resulted in an umbrella term, postbiotics, which refers to non-viable microbial cells, metabolic byproducts and their microbial components released after lysis. Postbiotics, modulate immune response, gene expression, inhibit pathogen binding, maintain intestinal barriers, help in controlling carcinogenesis and pathogen infections. Postbiotics have antimicrobial, antioxidant, and immunomodulatory properties with favorable physiological, immunological, neuro-hormonal, regulatory and metabolic reactions. Consumption of postbiotics relieves symptoms of various diseases and viral infections such as SARS-CoV-2. Postbiotics can act as alternatives for pre-probiotic specially in immunosuppressed patients, children and premature neonates. Postbiotics are used to preserve and enhance nutritional properties of food, elimination of biofilms and skin conditioning in cosmetics. Postbiotics have numerous advantages over live bacteria with no risk of bacterial transloc...
Biointerface Research in Applied Chemistry, 2021
Postbiotics, products, or metabolites secreted by living probiotic bacteria like teichoic acids, peptides, enzymes, peptidoglycans, polysaccharides, organic acids, and external cell proteins are said to be produced during the bacterial fermentation process. However, postbiotics may provide immunization, antioxidant, prevents inflammation, low cholesterolemic, antimicrobial, antagonistic obesity, contrast hypertensive, and diabetic retinopathy impacts. In the current review, we attempt to display the antimicrobial performance of postbiotics. In this regard, we considered microbial strains used as postbiotic sources and postbiotics as antimicrobial agents in food products. All databases such as Science Direct, Scopus, Pub Med, and Google Scholar were examined using the following keywords: “postbiotics”, “Antimicrobial activity”, “Anti-inflammatory”, and “Low cholesterolemic”. Further studies demonstrated that probiotics are fed special forms of fiber (prebiotic) molecules, indicating ...
Food production, processing and nutrition, 2024
In recent decades, consumers, manufacturers, and researchers have been more interested in functional foods, which include probiotics, prebiotics, and postbiotics. Probiotics are live microbes that, when regulated in enough quantities, provide health benefits on the host, while the prebiotics are substrates that host microorganisms selectively use. Postbiotics are metabolites and cell-wall components that are beneficial to the host and are released by living bacteria or after lysis. Postbiotic dietary supplements are more stable than probiotics and prebiotics. Many bioactivities of postbiotics are unknown or poorly understood. Hence, this study aims to present a synopsis of the regular elements and new developments of the postbiotics including health-promoting effects, production, conceptualization of terms, bioactivities, and applications in the field of food safety and preservation. Postbiotics aid in bio preservation and the reduction of biofilm development in food due to their organic acids, bacteriocins, and other antibacterial activities. The present study examines the production of postbiotic metabolites in situ in food and the effects of external and internal food components. The antimicrobial roles, removal of biofilms, and its applications in preservation and food safety have also been discussed. This paper also explored the various aspects like manipulation of postbiotic composition in the food system and its safety measures.
Comprehensive Reviews in Food Science and Food Safety, 2020
There are many critical challenges in the use of primary and secondary cultures and their biological compounds in food commodities. An alternative is the application of postbiotics from the starter and protective lactic acid bacteria (LAB). The concept of postbiotics is relatively new and there is still not a recognized definition for this term. The word "postbiotics" is currently used to refer to bioactive compounds, which did not fit to the traditional definitions of probiotics, prebiotics, and paraprobiotics. Therefore, the postbiotics may be presently defined as bioactive soluble factors (products or metabolic byproducts), produced by some food-grade microorganisms during the growth and fermentation in complex microbiological culture (in this case named cell-free supernatant), food, or gut, which exert some benefits to the food or the consumer. Many LAB are considered probiotic and their postbiotic compounds present similar or additional health benefits to the consumer; however, this review aimed to address the most recent applications of the postbiotics with food safety purposes. The potential applications of postbiotics in food biopreservation, food packaging, and biofilm control were reviewed. The current uses of postbiotics in the reduction and biodegradation of some food safety-related chemical contaminants (e.g., biogenic amines) were considered. We also discussed the safety aspects, the obstacles, and future perspectives of using postbiotics in the food industry. This work will open up new insights for food applications of postbiotics prepared from LAB.
The claimed health benefits of fermented functional foods are expressed either directly through the interaction of ingested live microorganisms, bacteria or yeast with the host (probiotic effect) or indirectly as a result of ingestion of microbial metabolites produced during the fermentation process (biogenic effect). Although still far from fully understood, several probiotic mechanisms of action have been proposed, including competitive exclusion, competition for nutrients and/ or stimulation of an immune response. The biogenic properties of fermented functional foods result from the microbial production of bioactive metabolites such as certain vitamins, bioactive peptides, organic acids or fatty acids during fermentation.
Biomedical Journal of Scientific & Technical Research
Mini Review Mini Review The human gastrointestinal tract (GI-tract) is composed of a microbial habitat, representing the site with a dynamic and complex mutualistic relationship between the gut microbiota and the host. The gut microbiota enhances the multitude of physiological functions in host with this mutual communication; the host's immune system often mediates this role [1]. Individual representatives of the gut microbiota also yielding a wide range of components that can be utilized by the host as well as by some other microbial species present. These mutual associations identified as the host-microbe and microbial community intercommunications [2]. There are several ways known to modulate the functional as well as compositional aspects of gut microbiota. The term '-biotics' means a strategy applied to nutrition favoring the gut microbiota for the host health in a most favorable state. The 'biotic' is a term assigned from the Greek word bi¯otikós, mentions 'pertaining to life', which illustrates to the bioecological community composed of living organisms together with their physical environment [3].
Gastroenterology & Hepatology: Open Access
Mini Review Postbiotics are defined as "any factor resulting from the metabolic activity of a probiotic or any released molecule capable of conferring beneficial effects to the host in a direct or indirect way. 1 The term postbiotic was introduced to describe a product containing dead microorganisms and their metabolites such as soluble factors secreted by live bacteria or released after bacterial lysis, including enzymes, peptides, teichoic acids, cell surface proteins, polysaccharides, and organic acids. 2 Postbiotics are also known as: "Para probiotics", "Non-viable probiotics", "Inactivated probiotics", "Ghost probiotics", or "Metabiotic, biogenic". All are inactivated microbial cells, which, when administered in sufficient amounts, confer benefits to consumers. 1,3
Frontiers in Microbiology, 2014
Current Opinion in Biotechnology, 2002
Recent research in the area of prebiotic oligosaccharides and synbiotic combinations with probiotics is leading towards a more targeted development of functional food ingredients. Improved molecular techniques for analysis of the gut microflora, new manufacturing biotechnologies, and increased understanding of the metabolism of oligosaccharides by probiotics are facilitating development. Such developments are leading us to the time when we will be able to rationally develop prebiotics and synbiotics for specific functional properties and health outcomes.
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