Molecular conformation and filtration properties of anionic Ficoll
Shuvo Roy2010, American Journal of Physiology-Renal Physiology
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Abstract
The physiology of glomerular permselectivity remains mechanistically obscure, despite its importance in human disease. Although electrical contributions to glomerular permselectivity have long been considered important, two recent reports demonstrated enhanced glomerular permeability to anionic versus neutral polysaccharides. The interpretation of these observations is complicated by confounding of the effects of chemical modification on charge with effects on size and shape. In this report, neutral and anionic Ficoll are characterized by size-exclusion chromatography with online light scattering and viscometry and filtration through a highly defined anionic filtration membrane. Neutral and carboxymethylated Ficoll are nearly identical in size and conformation, yet carboxymethylated Ficoll is retained by an anionic membrane in excess of neutral Ficoll. This suggests that comparisons between clearances of neutral and carboxymethylated Ficoll may be a sensitive probe of electrostatic ...
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is an as.sistant in thi-Dcpartnii-nt ot I rolog y, Facultv of Mcdit inc. Universitv of Hannover. Fédéral Repub lii of Ck-rmany; Dr. Bergmann is a research assixiale witfi llie Qiicen F.lisaf)eth NIedIcal Foundation. Bru.ssels, Bclgium. Onlv Dr. I.anitx-rl atteiided ihe conférence. The aiilhors wisli to acknowlcdge the c(>llatx)ration ol the following individuals: F. Hanus. S. IVgof'f, and M. Van Damnie. for dcvelopnient of the network niodel of glomerular circulation; M. Lammens-Vcrslijpe. R. \'an Holder and J. Sennesael, for sieviiig studies on dogs; C:. .N'guven Huu and R. Du Bois, for development of the theorctical inodel of charge seicctivity; M. Landuyt for electrophoretic mob ilities of the DS fractions; and M. Doriaux for the morphological study of the rénal damage produced b v the inf usion of c.itionized seriim alb umin.
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Measurement of the Permeability of Biological Membranes Application to the glomerular wall
The Journal of General Physiology
The transport equation describing the flow of solute across a membrane has been modified on the basis of theoretical studies calculating the drag of a sphere moving in a viscous liquid undergoing Poiseuille flow inside a cylinder. It is shown that different frictional resistance terms should be introduced to calculate the contributions of diffusion and convection. New sieving equations are derived to calculate r and A,/Ax (respectively, the pore radius and the total area of the pores per unit of path length). These equations provide a better agreement than the older formulas between the calculated and the experimental glomerular sieving coefficients for [6I]polyvinylpyrrolidone (PVP) fractions with a mean equivalent radius between 19 and 37 A. From r and A,/Ax, the mean effective glomerular filtration pressure has been calculated, applying Poiseuille's law. A value of 15.4 mm Hg has been derived from the mean sieving curve obtained from 23 experiments performed on normal anesthetized dogs.
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